Immunofluorescence
 DyLight Antibodies and Streptavidin Conjugates
DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. In comparison, the DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence. Vector Labs have introduced these enhanced fluorescent compounds conjugated to affinity-purified secondary antibodies and streptavidin for use in a variety of applications in particular cell and tissue based immunofluorescent antigen detection/visualization.   The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red). The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. Furthermore, the DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  
!
!

 

DyLight 594 labeled Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

1 mg
DL-1177
!
dylight594-lel6s
Retina flat mount from mice, perfused with DyLight 594-LEL. The retina were mounted in Vectashield HardSet+DAPI. Image courtesy of George W. Smith, Florida Atlantic University. 
isolated from tomato fruit
Tomato lectin is a very stable glycoprotein containing about 50 percent arabinose and galactose. This lectin is composed of a single polypeptide of about 100,000 daltons that may form aggregates in solution. Like other lectins that bind N-acetylglucosamine oligomers, tomato lectin prefers trimers and tetramers of this sugar. Tomato lectin, although sharing some specificities with potato, Datura lectin and wheat germ agglutinin, has been reported to be dissimilar in many respects. Tomato lectin binds well to such glycoproteins as glycophorin and Tamm-Horsfall glycoprotein.
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.
DyLight® 594 conjugated tomato lectin provides an excellent contrast to green/yellow fluorescence such as GFP expressed in transgenic animals, or with fluorescein conjugates in standard double label studies. The tomato lectin complements our existing range of lectin reagents and should be a valuable tool in examining rodent tumor angiogenesis, tracing neovascular development in xenograft models and brain research.
Inhibiting/Eluting Sugar: Chitin Hydrolysate (Cat. No. SP-0090)