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Agarose Avidin D,
A-2010
| Agarose Avidin D is prepared by conjugating Avidin D to heat stable, cross-linked 4% agarose beads. The procedure we have developed for coupling Avidin D to agarose optimally preserves the biotin binding activity of the Avidin D. Unlike cyanogen bromide coupling, our procedure does not produce conjugates which can be leached from the gel with solutes such as Tris buffer or generate charged groups on the gel that can bind proteins nonspecifically. Our Agarose Avidin D is coupled at 2 mg Avidin D per ml of gel and has a binding capacity of approximately one mole of biotinylated protein per mole of Avidin D. |
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This product can be used to separate biotinylated macromolecules from unbiotinylated materials. One important use is to determine the percentage of proteins containing an accessible biotin following a biotinylation reaction. After removing unreacted biotin, an aliquot of the biotinylated protein is passed through a small column containing Agarose Avidin D. The amount of the protein binding to the column is a measure of the percentage of protein molecules with accessible biotin residues.
PROTOCOL | !
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5 ml
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