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Datura stramonium lectin (DSL), Unconjugated
L-1180
| isolated from Datura stramonium (Thorn Apple or Jimson Weed) seeds |
| This 86,000 molecular weight glycoprotein contains two chains of 40,000 and 46,000 daltons joined by disulfide bonds. This lectin is free of a reported 32,000 molecular weight contaminant protein. The carbohydrate binding site recognizes (b-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. |
| DSL is mitogenic toward human peripheral lymphocytes and its potency has been reported to increase as the lectin aggregates to larger molecular weight species. Datura lectin also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (b-1,6) and (b-1,2) was reported to be the most potent inhibitor of agglutination. Carcinoembryonic antigen, capsular polysaccharides and many glycoproteins have binding sites for this lectin. |
| Inhibiting/Eluting Sugar: Chitin Hydrolysate (Cat. No. SP-0090) |
MSDS !
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5 mg
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