AMCA Avidin D is produced by conjugating Avidin D with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence. Most paraffin-embedded tissues have little autofluorescence under those conditions. AMCA Avidin D may provide an advantage in many labeling situations over conjugates of fluorescein and related dyes. AMCA Avidin D can be used in frozen or paraffin-embedded sections as a single label or in combination with other fluorescent conjugates for multiple labeling studies.
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